Tutor profile: Charles Y.
What is the difference between the counting unit "一匹“ (Yi pi) and "一隻” (Yi Zhi) when counting animals?
Well counting animals can often be confusing in terms of the counting unit that should be used, the general rule of using which one is by examining the use of the animal in the past. Animals such as houses, cows, sheeps, etc. use "一匹", since they can either be ridden or used for farming purposes. To better visual this, the character "匹" can be visualized as a saddle, which people would put on the aforementioned animals. Meanwhile, while the unit "一隻" can be applied to wider range of animals, it is usually applied to animals that excepted the ones aforementioned.
Subject: Biomedical Science
The nodes of Ranvier are interruptions in the myelin sheath in myelinated fibers, and typically they are located about 1 mm apart along the length of the axon. In saltatory conduction, what “jumps” from node to node?
In saltatory conduction, the action potential "jumps" from node to node, even though this phenomenon is actually caused by extremely rapid signal conduction inside the myelinated portion of the axon. When the cation (sodium ions) rushes into the cytosolic space from the extracellular space, an electrical force is created that pushes the cations inside the axon along the myelinated portion. This rapid conduction by the electrical force then reaches the next node to create another action potential, which then initiate another signal induction at the next node.
'm trying to understand a molecular mechanism of how pTAC-MAT-Tag-1 vector predigested with HindIII and EcoRI can be recircularized.
Since the cut sites of HindIII and EcoRI lead to sticky ends in the cut vector, which HindIII leads to 5'-AGCT-3' and EcoRI to 3'-TTAA-5', the linearized vector will have base pairing at the A and T at the ends. Thus it is more likely to recircularized after the vector is digested.
needs and Charles will reply soon.