Tutor profile: Cecil H.
Subject: Biomedical Science
How does an SDS-PAGE gel separate proteins
First proteins are mixed with the detergent SDS. This coats the proteins evenly and provides a uniform charge for proteins of similar sizes. Next, the protein/SDS mixture is loaded into an acrylamide gel. An electrical current is then applied and the protein enters the polymer, which then acts like a filter and separates by size of the proteins, with the smaller proteins near the bottom of the gel and the larger proteins at the top.
What are the steps required to break glucose down fully. What are the intermediates created at each step?
There are 4 distinct steps during standard glucose metabolism 1. Glycolosis, which generates 2 pyruvate, 2 NADH, and 2 ATP 2. The Citric Acid Cycle, which generates 6 NADH, 2 FADH2, 4 CO2, and 2 ATP 3. Pyruvate dehydrogenase, which converts the 2 pyruvate into 2 acetyl-CoA and 2 CO2 4. The electron transport chain, which generates 34 ATP.
Explain the differences between mitosis and meiosis.
Mitosis is a process that is used when a cell is ready to divide into two identical daughter cells. These are both 100% identical in terms of genome. The process for this involves, replication of the chromosomes than an equal division of the chromosomes. Meiosis is used by specialized cells to produce gametes (sexual reproduction cells). This is done in a process similar to mitosis, using similar machinery, However, meiosis (the second round at least) does not undergo chromosome replication. Instead it splits the chromatids evenly amongst the two cells, generating two haplotype gametes.
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