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# Tutor profile: Johanna T.

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Johanna T.
Medical Laboratory Technologist with Alberta Precision Labs
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## Questions

### Subject:Applied Mathematics

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Question:

In molecular biology Tris-EDTA (TE) buffer is commonly used as a pH buffer and to help chelate cations such as Mg2+ and Ca2+. A 20 mmol/L stock solution of TE buffer is available but you only need 100 mL or 10 mmol/L TE buffer. Calculate a way to make this solution?

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Johanna T.

Using the (c=concentation, v=volume) C1V1 = C2V2 you can easliy calculate this. C1 = 20 mmol/L V1 = unknown C2 = 10 mmol/L V2 = 0.100 L (L will be necessary for the conversion) 20(x) * (10)(100) To solve for x you would get 0.05L or 50 mL Therefore 50 mL of the stock solution with 50 mL of deionized water will make 100 mL of the 10 mmol/L TE buffer.

### Subject:Clinical Lab Sciences

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Question:

Ensuring your own safety is of the utmost importance in the laboratory. With this knowledge what equipment would you wear when working with hazardous and biohazardous material. Where can you find this information?

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Johanna T.

The most important equipment is your personal protective equipment (PPE). Generally, this includes a gown, gloves, close-toed shoes, long pants and safety goggles. Some materials require other safety equipment and that information can be found in the Materials and Safety Data Sheet (MSDS).

### Subject:Anatomy

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Question:

Homeostasis is our bodies tendency towards a state of equilibrium. It's how our bodies regulate our internal conditions despite the external conditions. Using this knowledge please describe why our bodies produce fever and chills when we are sick.

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Johanna T.

Our body temperature is regulated by the hypothalamus (found at the base of our brain). Pyrogens are substances that induce fever and can be exogenously (bacterial cell lipopolysaccharide) or endogenously (cytokines produced by activated immune cells) produced. A pyrogen's presence causes the release of prostaglandin E2 (PGE2) which acts on the hypothalamus. Through this, the hypothalamus attempts to increase body temperature through retaining and producing heat (ie shivering). This ultimately leads to fever and chills.

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