Enable contrast version

Tutor profile: Lokesh B.

Inactive
Lokesh B.
Scientist Drug discovery
Tutor Satisfaction Guarantee

Questions

Subject: Biochemistry

TutorMe
Question:

How many different types of gametes are possible with following genotype AbDdEe

Inactive
Lokesh B.
Answer:

Eight types of gametes is possible. Type of gametes can be calculated by 2n (2 the power n), where n is the number of gene in heterozygous condition

Subject: Basic Chemistry

TutorMe
Question:

Suppose you are involved in analyzing the gene expression pattern of certain genes in the breast cancer cell line. Using RT-PCR and western blot analysis, you demonstrated that gene X is up-regulated in all breast cancer cells as compared to the non-cancerous cell line. Based on RT-PCR and western blot data, your supervisor hypothesized that gene X is an oncogene. You have given the assignment to validate this hypothesis. What would be your experimental design to validate this hypothesis?

Inactive
Lokesh B.
Answer:

Answer: All cancer cells share certain traits such as uncontrolled proliferation and the ability to metastasize. These traits are governed by the oncogene. The oncogenic nature of gene X can be examined by following approaches. Gene X will be cloned and expressed in a non-cancerous cell line. If gene X is Oncogenic, a non-cancerous cell will begin to behave like cancerous cell and exhibits uncontrolled cell growth and metastasis. Uncontrolled cell growth can be examined by MTT assay, whereas metastasis ability can be examined by the wound-healing experiment. Another approach to demonstrate the oncogenic nature of gene X is as follows. Gene X will be knockdown in cancer cells using the anti-sense RNA or CRISPR-Cas9 method. If gene X is Oncogenic, cancer cell would lose the ability for uncontrolled proliferation or the ability to metastasize.

Subject: Biology

TutorMe
Question:

Let's assume you received two DNA samples: DNA A and DNA B. One of DNA contains promoter sequences. Design an experiment that allows you to find out which DNA samples contain the promoter sequences. Assume you have access to all the required reagents (like primers, polymerase enzyme, dNTP, plasmids, etc.) and resources to execute the experiments.

Inactive
Lokesh B.
Answer:

Luciferase reporter assay is the most accepted tool to identify and characterized the promoter sequence. Both DNA samples will be clone in pGL3 Luciferase reporter plasmid, individually. A reporter plasmid containing DNA A named as pGL3-A whereas, reporter plasmid containing DNA B named as pGL3-B. Mammalian cell line will be grown in 3 wells of six-well plate up to 70%–80% confluence. One well will be transfected with plasmid pGL3-A, the second well will be transfected with plasmid pGL3-B and third well will be transfected with vector alone pGL3 (served as a negative control). After incubation for a desired time, luciferase activity will be measured in each transfected sample. A DNA sample that contains the promoter sequence would show luciferase activity whereas a DNA sequence that does not contain promoter sequence would NOT show luciferase activity. For example, if we detect luciferase activity in a sample transfected with pGL3-A plasmid. It implies that DNA A contains a promoter sequence.

Contact tutor

Send a message explaining your
needs and Lokesh will reply soon.
Contact Lokesh

Request lesson

Ready now? Request a lesson.
Start Lesson

FAQs

What is a lesson?
A lesson is virtual lesson space on our platform where you and a tutor can communicate. You'll have the option to communicate using video/audio as well as text chat. You can also upload documents, edit papers in real time and use our cutting-edge virtual whiteboard.
How do I begin a lesson?
If the tutor is currently online, you can click the "Start Lesson" button above. If they are offline, you can always send them a message to schedule a lesson.
Who are TutorMe tutors?
Many of our tutors are current college students or recent graduates of top-tier universities like MIT, Harvard and USC. TutorMe has thousands of top-quality tutors available to work with you.
BEST IN CLASS SINCE 2015
TutorMe homepage
Made in California by Zovio
© 2020 TutorMe, LLC
High Contrast Mode
On
Off