Tutor profile: Vanshika N.
You are given a mystery substance with the melting point of 122.5-124.5 degrees Celsius. Substance A has a melting point range of 123-125 degrees Celsius and substance B has a melting point range of 122-124 degrees Celsius. How can you tell what the mystery substance is?
To find out what the mystery substance is, mix substance A and substance B individually with the mystery substance. Then, take the melting point of the two new mixtures. The mixture that has a higher and narrower boiling point, is the pure substance, and therefore, is the mystery substance. This is because impurities cause boiling point to be lowered and widen. For ex: if substance A + mystery substance led to a melting point range of 123-124.5, but substance B + mystery substance lead to a melting point range of 118-121, it means that substance B is an impurity in the mystery substance and thus the mystery substance has to be substance A.
What is the Big 5 Personality Theory?
The Big 5 Personality theory states that personality can be divided into the following 5 characteristics, which then have certain global and specific factors attached to them: Openness - how open you are to new experiences Conscientiousness - how organized/careful/extra thoughtful you are Extraversion - how outgoing are you/how much do you like to be surrounded by others Agreeableness - how friendly and compassionate are you Neuroticism - how nervous or insecure are you
Please explain the process of DNA replication and the enzymes involved.
The first step in DNA replication is to use the enzyme DNA Helicase to unzip the DNA molecule and break the hydrogen bonds between the nucleotide base pairs. Then DNA Topoisomerase helps to stabilize the unwound DNA strands, which then allows a replication fork to be formed (in the shape of a Y). The strand that is going from 3' to 5' is the leading strand and the other is the lagging strand. On the leading strand, RNA primer is able to bind once and allows for the start of DNA synthesis, which is carried out by DNA Polymerase III along the length of the strand. On the lagging strand, multiple RNA primers are bound along various lengths of the strand, as the DNA synthesis occurs in the opposite direction of how the DNA is being unzipped. This causes Okazakki fragments. These fragments are then put together with DNA Ligase. Then, to finish of replication DNA Polymerase I comes and replaces all of the RNA primers that were placed down with DNA.
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