Tutor profile: Zach S.
Define the pKa and explain its importance.
The pKa quantitatively describes the acidic strength of a particular reaction. Molecules can have multiple possible reactions, each with its own pKa. The pKa of a reaction can be calculated via the following equation: Ka= [A-][H+]/[HA] given the chemical equation of HA -- A- + H+. The pKa of a reaction is important in fields such as organic chemistry and biochemistry when it is important the determine the degree of protonation vs. deprotonation of a particular organic group. Given the surrounding pH and the pKa of the group, the % protonation can be explicitly calculated.
Define the 4 levels of protein structure.
Primary - Order of amino acids. Secondary - Local sub-structures within the protein chain, often α-helix and β-strand. Tertiary - Three dimensional folding of individual or multimeric protein strands. Quaternary - Three dimensional folding of multiple distinct polypeptide chains.
When would you use a Student's t-test?
The Student's t-test, or just t-test, is most often used to compare two sets of data and determine if they are significantly different than each other. The t-test is not used to determine the difference between more than two sets of data. The output of the t-test is a p-value, which indicates the likelihood of the null hypothesis being true.
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