Tutor profile: Jessica K.
Subject: Inorganic Chemistry
Reaction A has the following scheme: A + B ---> 2 C At a constant temperature, when the concentration of A is doubled, the reaction rate quadruples. When the concentration of B is doubled, the reaction rate doubles. What is the reaction order with respect to each reactant and the overall reaction order for Reaction A?
The reaction order with respect to reactant A is 2, meaning the rate is proportional to the square of [A]. The reaction order with respect to reactant B is 1, meaning the rate is directly proportional to [B]. The overall reaction order is 3 (third order) which is the sum of the two individual orders.
Protein A is marked at 120 kDa on a native gel. When run on a non-reducing SDS-PAGE, 1 band at 120 kDa appears and when run on a reducing SDS-PAGE, 1 band at 60 kDa appears. What is the quaternary structure of the protein and what type of bonds hold its subunits together?
Protein A is a homodimer with its subunits linked by disulfide bonds. On a native gel, no denaturing or reducing agent is present so a protein will keep its 3D conformation and quaternary structure. When only a denaturing agent (SDS) is added to the gel, the 120 kDa protein remains intact, meaning the subunits are not held together by weak intramolecular interactions such as hydrogen bonding. However, when denaturing and reducing agents are added to the gel, the protein separates into 2 identical subunits, indicating the dimer subunits are held together by disulfide bonds which are broken when reduced.
A patient comes into the ER complaining of a pain in their right upper quadrant, weight loss, jaundice, nausea, and dark urine. What is the structure that is likely blocked and what is it's normal function in the body?
Based on the location of the pain and jaundice, the common bile duct is most likely obstructed. This duct normally delivers bile stored in the gallbladder (produced in the liver) into the duodenum for lipid digestion. When blocked, bilirubin, a waste-product found in bile, builds up in the bloodstream and results in jaundice.
needs and Jessica will reply soon.