Tutor profile: Jonathon L.
Subject: Clinical Lab Sciences
Why is it important to run gel electrophoresis from the negative pole to the positive pole?
DNA has an inherent negative charge. This means it is repelled by other negatively charged objects and attracted to positively charged objects. By running the gel negative to positive, the DNA is pulled through the gel towards the positive node at the bottom of the gel. This allows the DNA to be separated out on the gel to be viewed or extracted.
What is the most important metabolite in the human body and why?
The most important metabolite in the human body is Acetyl-CoA. Glucose is broken down to produce acetyl-CoA in the mitochondria, cytoplasm, and many other organelles. Acetyl-CoA is a central metabolite involved in a vast amount of metabolic pathways. It is used in the mitochondria to produce ATP, in the cytoplasm for fatty acid production, in the nucleus for histone and other protein acetylation, and many more.
Why is it important for DNA replication to be imperfect? Why would our cells make mistakes when replicating DNA?
The fact that cells sometimes make errors when replicating DNA is extremely important for evolution and adaptation. By introducing errors into DNA, cell replication is changing and adding new traits to a species. This allows a species to continuously adapt to its environment by propagating the new traits that are best suited for the current environment. Without DNA replication error, life would stop existing.
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