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Kim B.
Molecular Biology and Neuroscience Research Student!
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Biology
TutorMe
Question:

A geneticist is creating a novel mutant cross using drosophila as their choice of model organism. Their two animals have chromosomal makeup as follows: Individual 1: +; P{w[+mC]=GAL4-elav.L}2/CyO;+ Individual 2: w[*];+;P{w[+mC]=UASp-Wee1.VFP}3 1a - Which of the two individuals being used in this novel mutant cross carries a phenotypic marker? 1b - How is this phenotypic marker denoted in the chromosomal makeup? 1c - Which chromosome is the marker located on? 2a - Consider the potential offspring from this novel mutant cross. How many different potential genetic makeups may be seen? 3c - What are the expected relative frequencies of the potential genetic makeups?

Kim B.
Answer:

1a - Individual 1 carries the phenotypic marker. In addition to recognizing the presence of a known mutation, we can also determine the presence of the marker being on individual 1 due to individual 2 showing a full homozygous makeup (i.e. each chromosome has two copies of each allele being looked at). Understanding that all phenotypic markers are lethal when two copies are carried, we can rule out this individual as carrying this marker. 1b - This phenotypic marker is denoted as "CyO" for the mutation Curly Wing. 1c - This chromosome is located on chromosome two of individual 1. 2a - Individual 1 is homozygous for chromosome 1 and chromosome 3, but heterozygous for chromosome 2. Individual 2 is homozygous for chromosome 1, 2, and 3. Creating a punnet square of the potential combinations will bring an individual to the conclusion that there are two potential genetic makeups, which would be as follows: Potential Genetic Makeup 1: +/w[*]; P{w[+mC]=GAL4-elav.L}2/+; +/P{w[+mC]=UASp-Wee1.VFP} Potential Genetic Makeup 2: w[*]/+; CyO/+; +/P{w[+mC]=UASp-Wee1.VFP} 2b - Two distinct potential genetic makeups mean that statistically the ratio of the two will be infinitely approaching 50:50

Anatomy
TutorMe
Question:

What two ions are the key players in determining the resting membrane potential of a neuron? Are these ions positively or negatively charged?

Kim B.
Answer:

Sodium (Na) and Potassium (K) are the two ions which play the largest role in determining the resting membrane potential of a neuron. Sodium is positively charged and potassium is negatively charged.

Algebra
TutorMe
Question:

Jay trying to do some math and he is stumped! If Jay knows the sample he was testing was multiplied by 1,000 and the machine says the cell count in the test tube after being multiplied was 43,000, how many cells were in the test tube when Jay started?

Kim B.
Answer:

If Jay knows that the test tube contained 43,000 cells at the end of his experiment, and he knows that the machine multiplied his sample by 1,000, Jay can calculate the number of cells he had in the test tube initially by using the algebraic expression: 1000x = 43000, where x = the number of cells in the test tube before the experiment. 43000/1000 = 43, meaning Jay had 43 cells in his test tube at the beginning of his experiment.

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